Table of Contents
by Jade Aster T. Badon and David J. Lohman
Philippine Journal of Systematic Biology, Volume 14, Issue 3, 2020, DOI: 10.26757/pjsb2020c14001
Abstract (Short Communication)
Date Posted (Final Published Version) :August 12, 2020
Recent curation of the butterfly collections in the National Museum of Natural History in Manila, Philippines, and the McGuire Center for Lepidoptera and Biodiversity, Florida Museum of Natural History, University of Florida, USA, resulted in new island records of Ideopsis juventa luzonica (Nymphalidae: Danainae) from Mabog Island in the Babuyan Islands, Philippines, Cheritra freja pallida (Lycaenidae: Theclinae) from Sebuku Island, Borneo, and Eurema hiurai (Pieridae: Coliadinae) in Zambales (Luzon) and Cotabato (Mindanao), Philippines.
KEYWORDS: butterflies, range, Philippines, Indonesia
by Vanessa Kate I. Alvarez, Ireneo L. Lit, Jr., Cristian C. Lucañas, Kristine O. Abenis, Romnick A. Latina, and Barbara L. Caoili
Philippine Journal of Systematic Biology, Volume 14, Issue 3, 2020, DOI: 10.26757/pjsb2020c14002
Abstract (Primary Research Paper)
Date Posted (Final Published Version) :August 25, 2020
The mitochondrial cytochrome c oxidase subunit 1 (COI) nucleotide sequences of Unaspis mabilis Lit & Barbecho (Hemiptera: Diaspididae), are provided for the first time. The total genomic DNA of each scale insect was extracted from individuals infesting lanzones leaves from three selected sites in Los Baños, Laguna. A partial COI gene amplicon with approximately 750 bp was obtained using the primer pair PcoF1 and LepR1. Nucleotide sequence alignment showed no variation among the COI sequences from all the samples. BLASTn search yielded no significant match with any of the available sequences for Unaspis species. The closest hit was Aulacaspis tubercularis Newstead (GenBank Accession No. HM474091) with 87.4% nucleotide similarity. Nonetheless, phylogenetic analyses revealed that generated COI sequences from U. mabilis form a monophyletic clade with U. yanonensis and U. euonymi, with closer proximity to the former. These findings also strengthen the species status of U. mabilis under the genus Unaspis. The DNA barcodes generated from this study (GenBank Acc. Nos. MN114099, MN14101, and MN114102), could, therefore, be used to verify the species identity of other lanzones scale accessions, as well as monitor the distribution and spread of U. mabilis, which would greatly influence possible pest management options.
KEYWORDS: cytochrome C oxidase I, COI, Lansium domesticum Correa, lanzones
by Harold B. Lipae, Angelique L. Estabillo, Ian Kendrich C. Fontanilla, and Emmanuel Ryan C. de Chavez
Philippine Journal of Systematic Biology, Volume 14, Issue 3, 2020, DOI: 10.26757/pjsb2020c14003
Abstract (Primary Research Paper)
Date Posted (Final Published Version) : September 20, 2020
A new subspecies of microsnail, Hypselostoma latispira masungiensis subsp. nov., is described based on shell morphology and molecular characters. This new subspecies is distinguished from H. l. latispira from Baguio City, Benguet Province by having relatively larger major width size, additional apertural teeth (interpalatal plica), larger body whorl and apertural width, and clustering based on location. The collected samples from Masungi Georeserve, Rizal Province appear to be an ecophenotype as indicated by the novel site congruent to the clade separation of Masungi and Baguio H. latispira. Neighbor-joining and maximum likelihood trees also demonstrated that the two sample groups clustered separately, with bootstrap support of 84% and 78%, respectively. However, pairwise distance comparison revealed that there is only an average of 0.0131 ± 0.0126 genetic distance (99.98%) between the two populations, suggesting that they are most likely similar species; thus, the proposal of making it a subspecies. This is the first report on the new distributional record outside the type locality and a new subspecies of H. latispira.
KEYWORDS: land snail, karst, interpalatal plica, pairwise distance comparison